This fifth edition of the successful, long-selling classic has been completely revised and expanded, omitting some topics on obsolete DNA electrophoresis, but now with a completely new section on electrophoretic micro-methods and on-the-chip electrophoresis.
The text is geared towards advanced students and professionals and contains extended background sections, protocols and a trouble-shooting section. It is now also backed by a supplementary website providing all the figures for teaching purposes, as well as a selection of animated figures tested in many workshops to explain the underlying principles of the different electrophoretic methods.
Part I Fundamentals
Chapter 1 Electrophoresis
Chapter 2 Isotachophoresis
Chapter 3 Isoelectric Focusing
Chapter 4 High-Resolution Two-Dimensional Electrophoresis
Chapter 5 Protein Sample Preparation
Chapter 6 Protein Detection
Chapter 7 Blotting
Part II Equipment and Methods
Chapter 8 Special Laboratory Equipment
Chapter 9 Consumables
Chapter 10 Chemicals
Method 1 PAGE of Dyes
Method 2 Agarose and Immunoelectrophoresis
Method 3 Titration Curve Analysis
Method 4 Native PAGE in Amphoteric-Buffers
Method 5 Agarose IEF
Method 6 PAGIEF in Rehydrated Gels
Method 7 Horizontal SDS-PAGE
Method 8 Vertical PAGE
Method 9 Semidry Blotting of Proteins
Method 10 IEF in Immobilized pH Gradients
Method 11 High-Resolution 2D Electrophoresis
Method 12 PAGE of DNA Fragments
